Abstract
Aim
This study aimed to investigate the rate of Panton-Valentine Leukocidin producing
Staphylococcus aureus and methicillin (mecA) and slime (icaA/icaD) genes in staphylococcal strains isolated from nasal cavities of footballers.
Materials and Methods
Nasal swab samples were taken from each footballers and a healthy control group for
the isolation of staphylococcal strains. The polymerase chain reaction technique was
used to determine Panton-Valentine Leukocidin, mecA and icaA/icaD genes in staphylococcal isolates.
Results
Among 91 S. aureus strains, the presence of mecA gene was detected as 9.9%. This ratio was 17.9% (27 of 151) among the coagulase-negative
staphylococci. A significant difference was found between coagulase-negative staphylococci
and S. aureus isolates regarding the presence of mecA gene (P < 0.001). As for the genes of the slime, icaA/icaD genes were detected in 198 of 242 (81.8%) strains. The occurrence of slime genes
was 91.2% and 89.4% among the S. aureus coagulase and negative staphylococci, respectively (P > 0.05). There was a statistically significant difference between the frequency of
the mecA and slime genes when compared with the healthy control group and the football players
(P < 0.01). Of 91 isolates, 22 were found to be methicillin resistant by the oxacillin
disc diffusion method, whereas the remaining (220) were methicillin susceptible. Methicillin
resistance was detected as 14.9% by the polymerase chain reaction method, whereas
it was found as 9.1% by phenotypic methods.
Conclusions
Early and accurate diagnosis of virulent staphylococcal strains is crucial because
the virulent coagulase-negative and coagulase-positive staphylococcal strains in the
nasal floras of footballers may be major potential sources of superficial and deep
tissue infections.
Key Indexing Terms
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Article info
Publication history
Accepted:
November 5,
2015
Received:
August 3,
2015
Footnotes
☆The article has not been submitted elsewhere, is not under review, or published previously, except in abstract form. Its abstract published in I. International Physical Activity, Nutrition and Health Congress, November 23-26, 2011, Antalya, Turkey.
☆☆The authors have no financial or other conflicts of interest to disclose.
Identification
Copyright
© 2016 Southern Society for Clinical Investigation. Published by Elsevier Inc. All rights reserved.
